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Be certain that all the purification buffers are at 4 °C and perform the protein purification within the cold room to avoid protein denaturation. OBSERVE: This avoids the coupling of amino acids in the further cycles with the membrane as an alternative of the growing peptide chain. NOTE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino groups between the spots and likewise a number of the amino teams inside the spot areas don't kind bonds with the amino acids. |
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The members of the SUV39 family discussed above are concerned in each euchromatin and heterochromatin, however one other member of the identical family, G9a, is the predominant histone H3 K9 methyltransferase in mammalian euchromatin 14 There are two isoforms of G9a in the mouse: the quick form (GenBank accession number NP_671493 ) corresponds to human G9a and the lengthy type ( NP_665829 ), which lacks intron one, has additional Arg-Gly repeats at the amino terminus. |
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